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The major challenge of antibody detection is its inability to differentiate between active and past infections because of the persistence of antibody in serum or other samples after cure, which is demonstrated by the low specificity of serological tests in those individuals from endemic areas and ingesting raw freshwater fish (196, 199)

The adjustment period typically lasts two to four weeks, during which mild side effects may occur before resolving

The open end of each straw was sealed, and the straws were cooled in liquid nitrogen vapor for 10 s

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