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cDNA was diluted tenfold with water and quantitative PCR assays were performed in a StepOnePlus real-time PCR system (Applied Biosystems, Waltham, MA) using TaqMan chemistry

Rodgers, J

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Analyses of essential glutathione-associated enzymes in susceptible and resistant responses have demonstrated that the levels of enzymatic activity or the absence of specific enzymes can impact the spread of the virus and activate host-induced defense mechanisms

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Once reconstituted with bacteriostatic water, store refrigerated and use within 2 to 4 weeks