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The digested peptides were reconstituted in 2% acetonitrile/0.1% TFA and 5 l of each sample was loaded onto a PepMap 100 3U 75 m 20 mm reverse-phase trap where they were desalted online before being separated on a 100 2U 50-micron 150 mm PepMap EasySpray reverse-phase column 17

And actually that's small density

Four-Octyl Itaconate Protects Chondrocytes against H(2)O(2)-Induced Oxidative Injury and Attenuates Osteoarthritis Progression by Activating Nrf2 Signaling

Int.J Cosmet.Sci 2011;33(6):519-526