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After incubation, mitochondria were pelleted (1 min at 16,000 g ) and pellets were resuspended in 200 L of 50 mM HEPES buffer (pH 7.4) supplemented with 10 mM IAM-TPP, disrupted using mechanical micro-homogeniser and sonicated for 5 min

Fact: While the body does produce Glutathione, levels can be depleted by stress, poor diet, pollutants, and the natural process of ageing

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Step 5: Observation: The expert monitors you for any reactions and provides aftercare guidance

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