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Jumper J, Evans R, Pritzel A, Green T, Figurnov M, Ronneberger O, Tunyasuvunakool K, Bates R, Zidek A, Potapenko A, Bridgland A, Meyer C, Kohl SAA, Ballard AJ, Cowie A, Romera-Paredes B, Nikolov S, Jain R, Adler J, Back T, Petersen S, Reiman D, Clancy E, Zielinski M, Steinegger M, Pacholska M, Berghammer T, Bodenstein S, Silver D, Vinyals O, Senior AW, Kavukcuoglu K, Kohli P, Hassabis D (2021) Highly accurate protein structure prediction with Alphafold

The following primers were used: AgRP (forward: ctttggcggaggtgctagat

Equivalent amounts of protein (20 g) from each sample were subjected to electrophoresis on a SDS-polyacrylamide gel and were then transferred onto polyvinylidene difluoride membranes for subsequent blotting with specific antibodies

For chromatographic separation, a hydrophilic interaction liquid chromatography (HILIC) column (Acclaim Trinity P2, 3 m, 3.0 100 mm, Thermo Scientific) was used

The insulin-like growth factor I receptor/insulin receptor tyrosine kinase inhibitor PQIP exhibits enhanced antitumor effects in combination with chemotherapy against colorectal cancer models

As with any supplement, don't exceed the recommended dose