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After twice washing with PBS, the nuclei were counterstained with 10 g/ml DAPI (4,6-diamidino-2-phenylindole) (C0060, Solarbio) for 10 min at 37C

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Many patients find tirzepatide helpful when previous weight loss methods or medications were not effective enough

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No receptor-mediated adverse events (e.g., cardiovascular effects, endocrine disruption) have been reported, consistent with NNMT's limited expression outside metabolic tissues

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