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Correct insertion yields a novel NcoI site generated by ligation of the blunted ends, and so NcoI (NEB) linearization of the supercoiled vector was used to determine correct insertion (Figure 1b, lower panel)
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These include weight gain, fluid retention, increased appetite, mood changes, elevated blood pressure, increased infection risk, osteoporosis, skin thinning, and metabolic disturbances including hyperglycaemia
ParkBBakbakETeohHKrishnarajADennisFQuanAet al
PMID 26819561
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