Cell pellets were resuspended in 0.3 ml small-scale lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 1 mg ml 1 lysozyme, 0.75 mg ml 1 polymyxin B, 0.01 mg ml 1 DNase I) per well and incubated at 30 C, 300 rpm, 5 cm shaking diameter for 30 min
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Glutathione is often referred to as the bodys master antioxidant
Women with higher follicular glutathione levels have an increased fertilization rate when undergoing IVF
Exposure was accompanied by decreased cell viability, increased caspase 3 activity, and decreased intracellular GSH levels
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