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RNA extraction and quantitative RT-PCT (qRT-PCR) Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, USA) according to the standard RNA isolation protocol

Glutathione is a powerful antioxidant naturally produced in the body, crucial for cellular detoxification, immune function, and protecting against oxidative stress

J Exp Med 216(3):501516 Raina P, Kaur S (2012) Knockdown of LdMC1 and HSP70 by antisense oligonucleotides causes cell-cycle defects and programmed cell death in Leishmania donovani