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11242-1-AP, Proteintech, Rosemont, IL, USA) to observe the mitochondrial localization of CD147 and the binding of mito-CD147 scFv to mitochondrial CD147
Nanobody 35, excess peptide ligand (10 M), and apyrase were added to form a complex, which was solubilized in lauryl maltose neopentyl glycol (LMNG)/cholesterol hemisuccinate (CHS) mixed-micelles as previously reported
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