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Dourado, D
Cells were passaged into a new 6-well plate at a density of 1.2 10 6 cells per well, ~30 min before transfection in 2 mL of 2i medium and transfected with 250 L of Opti-MEM containing 8 L of Lipofectamine 3000 and 46 g of plasmid DNA
This 16-week titration period is essential
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Oxidative stress is damage caused by unstable molecules around us called free radicals, which are triggered by exposure to UV rays from the sun
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