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Am J Clin Nutr (2006) 84:517
DOI: 10.1073/pnas.1735466100 11
Kovacs-Nolan, J., Rupa, P., Matsui, T., Tanaka, M., Konishi, T., Sauchi, Y.,
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Companion satellite-cell cultures, prepared at the same time, were immunostained at 30-h post-plating with D3 anti-desmin mAb and DAB (a chromogenic HRP-substrate that forms an insoluble brown reaction product) to determine the percentage of myogenic cells present 18 ,19 cultures with less than 95% DAB-positive cells (namely, less than 95% desmin-positive myogenic purity) were not used for experiments just to ensure the reliability of data specific to myogenic cells in the employed primary cell culture systems
Therefore, high dosing of the drug in combination with other adjuvants is needed to effectively eradicate H
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