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These phenotypes were not attributed to tamoxifen treatment, as treatment of control mice ( Gclc f/w mice) with tamoxifen failed to cause any weight changes (Fig
In cases where the history is strong, an oral challenge may be deferred, and strict avoidance recommended
Migration scratch assay Cells were seeded (60,000 cells/well) in a 96-well plate (Greiner) coated with 0.3 mg/ml matrigel, allowed to attach overnight, and scratched using the IncuCyte Wound maker (Essen Bioscience)
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Hu et al., FAK and paxillin dynamics at focal adhesions in the protrusions of migrating cells, Sci
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