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Extinction coefficients were measured by creating a dilution series (115 M), measuring the absorbance at max and applying a linear regression
The nuclear protein fraction was extracted, and the relative Dnmt and 5-mC hydroxylase Tet activities were calculated based on the ratio of the treatment group to the control group after the normalization to the amount of protein
Brain Commun
The eIF2/PERK/ATF4 branch of the UPR also activates FGF21 under different conditions of ER stress, including change in ER redox state upon dithiothreitol treatment, or perturbation of ER calcium homeostasis with thapsigargin (156)
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(2022) 149:16281642.e10
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