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Tricyclic Antidepressants (TCAs) Amitriptyline and nortriptyline belong to an older class of antidepressants that remain highly effective for nerve pain

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But they work in very different ways, and choosing between them is not always straightforward

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The slides were washed in water/PBS and then blocked using 5% goat serum in PBS for 1 h at room temperature prior to adding primary antibodies diluted in the blocking buffer

Needle selection matters If you are drawing from a vial, you have control over needle selection