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Based on proximity labeling and co-immunoprecipitation, seven E3 ubiquitin ligases were identified as potential interactors of NHR-49, with the top candidates being the HECT domain-containing E3 ligase, HECD-1, with 30 PSMs detected via proximity labeling, and the WW domain-containing E3 ligase, WWP-1, with 110 PSMs identified through co-immunoprecipitation (Supplementary Fig

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Preparation of the medium The culture media used in the experiments were prepared according to the following specifications

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Cyanide inhibition of a 4-glutathione:4-selenoenzyme