After overnight incubation, cells were transfected with GIPR, GLP-1R or GCGR construct using Lipofectamine 2000 or Lipofectamine 3000 transfection reagent (Invitrogen)
R., Tabrizi, M., Ghaleiha, A., and Akhondzadeh, S
Consequently, changes in the ROS levels inside the cancer cells in a 1% O 2 atmosphere were examined by utilizing the ROS specific probe 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) which is chemically converted into a green fluorescent dye in the action of ROS
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