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Evidence in T1D is limited to small, short-term, randomized clinical trials (RCTs) and a few observational studies focusing on surrogate outcomes such as glycemic control and weight loss 8,9,10,11,12

These effects are most evident during treatment initiation and dose escalation, when nausea and vomiting tend to be more pronounced

Supernatants were assayed for total protein concentration with Bradford assay (BioRad 5000006) and 50100 g of protein was mixed with loading buffer and loaded in a 12 or 16% gradient gel (Thermo 1Fisher XP00162)

Avoid Chelators EDTA and other chelating agents will strip the copper ion from the peptide complex, rendering it inactive

from the University of Arizona