Behavioral satiety study Lean mice were fasted overnight before IP agonist injection
After centrifugation, the supernatants were mixed with Coomassie G-250 (SERVA) and loaded onto a native PAGE gel (Thermo Fisher Scientific)
Single-use aliquots can be frozen at 20C without the degradation risk that applies to multi-dose vials because they're only thawed once
B12 injections should be avoided during pregnancy, breastfeeding, or if you plan to become pregnant or breastfeed soon
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After 3 days: The dose may increase to 300 mg per day , taken as 150 mg twice daily , at least 8 hours apart