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After extensive washing with PBS and then NH 4 CO 3, bound proteins were eluted with 100 mM DTT, concentrated 5-fold and applied to 12% SDS-PAGE gels

Imaging studies indicating vagal atrophy and autonomic dysfunction in PD provide biological plausibility but do not prove directionality [133]

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The Aging Process Connection I began this research because I recognized that imbalanced hormones contribute to accelerated aging

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