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For transient expression, cells in 12-well plates were typically transfected at roughly 70% confluency using 2.5 l of polyethyleneimine (PEI

ITC measurements are complicated by the solubility of copper at the high concentrations required, where copper(II) tends to precipitate unless it is solubilized by weakly chelating buffer components

Starvation typically involves glycogen degradation, the utilization of lipids to support physiological maintenance, and a switch to protein degradation once the lipids are depleted 28

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This gene-level fingerprint helps explain why a single small peptide can produce such wide-ranging regenerative effects

Vitamin C is a powerful antioxidant that protects your cells from free-radical damage