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The potential role of ischaemia-reperfusion injury in chronic, relapsing diseases such as rheumatoid arthritis, Long COVID, and ME/CFS: evidence, mechanisms, and therapeutic implications

As both H 2 O 2 and BCNU exposure led to excessive cellular GSSG accumulation, their impact on intracellular protein-GSSG adduct formation (protein S -glutathionylation) was subsequently examined in HepG2 cells by western blot analysis using a monoclonal GSSG antibody that specifically binds to GSSG-modified proteins

Edwards CM, Todd JF, Mahmoudi M, Wang Z, Wang RM, Ghatei MA et al

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Organ culture bioreactors--platforms to study human intervertebral disc degeneration and regenerative therapy