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Specificity of staining was confirmed by staining with a rat IgG (6-001-F, R&D Systems, Bio-Techne), a Rabbit IgG (ab27478, Abcam) or a mouse IgG1, kappa (Abcam, ab18443) isotype controls
Stubborn fat that does not respond to diet and exercise is often a metabolic problem, not a willpower problem
When the body can't absorb nutrients, a deficiency may result
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