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cDNA was synthesized with the iScript cDNA Synthesis Kit (#170-8891, Biorad) and subjected to quantitative real-time PCR using SYBR Green Supermix (#172-5272, Biorad)

The choice between pen and vial is therefore above all a matter of how the reference material is stored and handled in the laboratory

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This is the warning sign for visceral organ growth, which is largely irreversible

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