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Once cells were proliferating, they were moved up to higher cell volumes and adapted to DMEM cell culture media with 20% dFBS, 50 U ml 1 penicillin and streptomycin and 1 mM sodium pyruvate for at least 1 week before experimental setup
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Binding of -MSH to MC4R triggers a Gs protein-coupled signaling cascade, which increases intracellular cyclic AMP (cAMP) levels and ultimately activates downstream pathways that promote satiety and increase energy expenditure [9]
References Arreola-Espino R, Urquiza-Marn H, Ambriz-Tututi M et al (2007) Melatonin reduces formalin-induced nociception and tactile allodynia in diabetic rats
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R., Hartoft-Nielsen, M.-L., Hansen, O
