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Check for Clarity: The final solution should be perfectly clear, just like water

Unconjugated bilirubin (UCB, i.e., indirect bilirubin) is absorbed actively or passively by hepatocytes, mediated by the hepatic organic anion transporting polypeptides 1B1 (OATP1B1), and passes through uridine glucuronosyltransferase family1 memberA1 (UGT1A1) undergoes mono- or bi-glucuronidation ( Figure 1 3 Effects of bilirubin on the body For a considerable time, bilirubin was regarded as a harmful metabolic waste

Crystals of HT adducts formed in a couple of days in 25% vol/vol PEG smear medium and 0.1 M Tris pH 8.5 (MeTRaQ-G), in 20% vol/vol PEG 6000, 10% ethylene glycol, 0.1 M magnesium chloride hexahydrate and 0.1 M MES pH 6 (TRaQ-G) and in 20% vol/vol PEG 6000, 0.1 M lithium chloride and 0.1 M sodium citrate pH 4 (TRaQ-G-ctrl)

Funding This research was supported by the China Postdoctoral Science Foundation (2021M701420), Guangdong Basic and Applied Basic Research Foundation (2022A1515110021), Guangzhou Science and Technology Projects Foundation (202201020002), Scientific Research Project of Guangdong TCM Bureau (20221105), Guangdong Medical Research Foundation (A2022214)

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