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Firstly, the permeability of EBA increased after 24-h incubation with 3-PLG-ibu, and secondly, in inflammatory condition besides the 3-PLG-ibu-A-GSH the free ibuprofen treated group also significantly strengthened the integrity of the BBB model for the bigger transcellular marker molecule (Fig

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Cells were then washed and resuspended in 200 cL flow cytometry staining buffer and analyzed using FACSAria III cell sorter (BD Biosciences, USA)