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Also, B12 levels are not generally checked during routine blood work

Although stress is also a factor which may influence insulin resistance, no doctor treating individuals with diabetes would consider that those needing higher doses of insulin are mentally unstable, making up their disease, or suffering from the placebo effect

Preclinical studies in diet-induced obese mice have examined its effects on adipose tissue and glucose tolerance

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Therefore, the optimized method was considered adequate for sample preparation and was further subjected to method validation