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Determination of Total Glutathione (GSH and GSSG) Concentration Determine the total glutathione concentration in the sample solution using the following equations

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Glycine: Small amino acid, big impact Glycine is the simplest amino acid in structure

In brief, the peak area of the metabolite was normalized to the peak area of an isotopically labelled internal standard with similar elution time, both in samples from culture medium and external standard library dilutions