Importantly, while recent genomic profiling indicates that some historically broad ovarian cancer cell lines (e.g., SKOV-3, A2780) may not strictly represent HGSOC, early foundational in vitro studiesparticularly those evaluating metastatic competenceheavily relied on these models
Vitamin C (40 mg) is a water-soluble antioxidant that supports immune function and collagen synthesis, and it can help regenerate vitamin E after it is used to neutralize free radicals
In order to obtain any reliable read-out in reasonable time, a low molecular redox mediator is almost regularly required to enable an electron transfer between the macromolecule and the electrode and, of course, access of oxygen has to be strictly prevented
The vote was advisory
Your cells - and your future self - will thank you.
Supernatant containing the protein portion was added with sample buffer (0.125 M Tris [pH 6.8], 10% [v/v] glycerol, 0.02% SDS, and 1 mM dithiothreitol [DTT]) and subsequently heated at 95 C for 5 min