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Proteins were separated on SDS/PAGE gels and electroblotted onto PVDF membranes (GE Healthcare, United States)
When stress induces neuroendocrine dysfunction(s), quite often, a hypoestrogenic condition occurs as a consequence of the ovarian blockade worsening the stress-induced abnormal neuroendocrine control of GnRH release from the hypothalamus On this basis, several studies have been done on the efficacy of opioid receptor antagonist on the restoration of a normal gonadotropin release and of menstrual cyclicity [3133]
Indole-3-carbinol: a plant hormone combatting cancer
