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Engineering nanomedicine for glutathione depletion-augmented cancer therapy
However, leakage of neither erythrocytes nor the low-molecular-weight tracer Sulfo-N-hydroxysuccinimide (NHS)-biotin was detectable in Kif11 cKO mice (Supplementary Fig
To generate plasmid pcDNA3.1/ARCHGEF11 coding for human PDZ RhoGEF for in vitro transcription-translation, PDZ RhoGEF was amplified by PCR by using plasmid mCitrine-YFP-ARHGEF11 as a template and inserted into the pcDNA3.1 zeo (+) vector (Invitrogen) in two steps: first, the nucleotides 11943 were inserted as a KpnI-EcoRI fragment and then the nucleotides 19444566 as an EcoRI-XhoI fragment
