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Subsequently, a circle was delineated around the tissue using a tissue-marking pen for histological assessment

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NTS prepared as previously described and cultured in M199 supplemented with 5.5 mM D-glucose, penicillin-streptomycin and 5% heat inactivated fetal bovine serum were incubated at 37 C in a clear bottom flat well plate in a 5% CO 2 incubator overnight