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doi: 10.1111/j.1399-0004.2011.01743.x 67
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After different groups of HUVECs are treated accordingly, they are seeded into 24-well plates pre-coated with 150 L/well growth factor-reduced Matrigel (Corning, catalog number 354234) and incubated at 37 C in a cell culture incubator for 12 h
These observations were further confirmed by Hoechst 33342 staining, which revealed extensive detachment of cells from the cell culture substratum and increases in apoptotic bodies of HepG2 cells incubated with H 2 O 2 and TNF for 16 h (Fig
So most people understand that excess weight causes excess fat around the airway, leads to obstructive sleep apnea
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