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Unlike fat-soluble vitamins (A, D, E, and K) that can build up, B12 has a strong safety record and toxicity is rare

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However, in guidelines published in 2012, the British Columbia Medical Association (Canadian Ministry of Health) recommended a dose of 1000 g/day for pernicious anemia or food-bound cobalamin malabsorption [34]

A 5mg vial is ideal for initial assay development, while a 15mg vial provides better economy for long-term wound healing models