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Its high selectivity and confirmed membrane permeability make it one of the most reliable small-molecule tools currently available for interrogating the NNMT pathway in both cell-based and in vivo experimental models

For instance, while not common, intramuscular injections of vitamin B12 have been reported to cause life-threatening anaphylaxis

Heavier individuals may require higher doses to achieve equivalent NNMT inhibition due to larger adipose tissue mass and potentially higher baseline NNMT activity

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