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2002), elevated concentrations of ROS cause oxidation as well as the loss of normal functions of many endogenous macromolecules such as lipids, nucleic acids and proteins, resulting in the development of various diseases (Mondola et al

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Pushing the plunger too fast forces the fluid into the tissue rapidly, creating pressure that causes stinging and can lead to lump formation

doi: 10.1038/nature13236 69 NilssonRJainMMadhusudhanNSheppardNGStrittmatterLKampfCet al

For local effects, many practitioners inject near the target area