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Louis, MO, USA) in a serial dilution from 400 to 0 M, followed by incubation at room temperature for 2 h, followed by removal of S-nitrosylglutathione (GSNO) through a buffer exchange using a PD10 column (Bio-Rad, Solna, Sweden)

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However, their effectiveness in achieving skin-lightening or similar benefits is still a subject of debate within the scientific community