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Briefly, the construct was produced by stable expression and secretion from CHO-K1 cells and then purified by a sequential combination of 9E10 antibody-affinity chromatography and size-exclusion chromatography as previously described 35 , with the final sample being prepared at a concentration of 0.2 mg mL 1 in a 24.8 mM Tris-HCl (pH 8.0), 137 mM NaCl, 2.7 mM KCl plus 0.02% NaN 3 (TBSA)

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Signs You May Benefit from Medically-Supervised GLP-1 Weight Loss Injections Diet and exercise aren't producing results Weight gain despite eating less Constant hunger and food preoccupation Previous weight loss attempts failed Regained weight after initial success Hormonal changes affecting weight Perimenopause or menopause weight gain Insulin resistance or prediabetes Thyroid dysfunction Metabolic slowdown in 40s+ If you're experiencing challenges listed above, GLP-1 weight loss injections with medical supervision could help you

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the lipidated GLP-1 molecules are bound to albumin, which is too large to be renally filtered, and are thus shielded from plasma proteases