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Methods Construct modification and expression of full-length GLP-1R The optimized gene of full-length GLP-1R was cloned into the modified pFastbac1 vector at the BamHI/HindIII sites and the native signal sequence was replaced by haemagglutinin (HA) to enhance receptor expression (see primers in Supplementary Table 2), followed by a flag-tag, a 10 His-tag and a tobacco etch virus protease (TEV) site at the N-terminus

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These therapies are designed to address the underlying hormonal and metabolic factors that influence weight

Apply a small amount of the product to a discreet skin area and monitor for reactions over the next 24 hours

Fructose stimulates GLP-1 but not GIP secretion in mice, rats, and humans