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Modulation by external calcium

If the room was a standard 70F, it is probably fine

Follow your institutions standard operating procedures for receiving, logging, aliquoting, labeling, and disposal

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35 In a burst fracture the intravertebral pressure increases and the bony fragments are pushed centrifugally apart, which weakens the anchorage of the annulus as well as creates gaps between the fragments to allow the nucleus to herniate into the underlying bone