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References: Stonehouse AH, Darsow T, Maggs DG

Marso SP, Bain SC, Consoli A, Eliaschewitz FG, Jodar E, Leiter LA, et al

By blocking NNMT, 5-Amino-1MQ drives several metabolic benefits: Boosts NAD Levels Inhibiting NNMT preserves more nicotinamide for NAD synthesis, leading to enhanced mitochondrial function, increased cellular energy output, and activation of sirtuins , the proteins involved in longevity and DNA repair

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Add 10 L Concanavalin A coated magnetic beads (Bangs Laboratories) and incubate it at RT for 10 min

Ziauddeen H, Alonso-Alonso M, Hill JO, Kelley M, Khan NA (2015) Obesity and the neurocognitive basis of food reward and the control of intake