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mRNA expression analysis by real-time PCR Total RNA was isolated from the dissected tissues using the RNeasy Mini kit (Qiagen, CA, USA), followed by reverse transcription using the High Capacity RNA-to-cDNA kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturers instructions

PMCID: PMC3991023

Glutathione is a tripeptide antioxidant composed of three amino acids glutamine, cysteine, and glycine synthesized endogenously in every human cell

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Linus Pauling and the common cold The person who popularized taking megadoses of vitamin C was Dr