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Complex was formed by adding 10 mM CaCl 2 , 10 mM MgCl 2 , 1 mM MnCl 2 , 50 mU/mL apyrase (Sigma-Aldrich, Darmstadt, Germany), 20 M GLP-2, 100 M TCEP (Sigma-Aldrich) and 10 g/mL Nb35 into the cell lysate and incubated at room temperature (RT) for 1.5 h
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10.1016/j.csbj.2025.04.011 Comput Struct Biotechnol J