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Cartilage presents unique challenges because it lacks direct blood supply and has limited self-repair capacity
To measure degradation, 10 l of a total concentration of 2.510 5 cells/ml in DMEM medium were seeded into white small volume 384 well plates (Greiner, 784075) and allowed to settle overnight
doi: 10.1155/2013/571420 21
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Blots were blocked with phosphate-buffered saline with 0.1% Tween 20 and then bound with antibodies for Biotin, after which an enhanced chemiluminescence (ECL) reaction was performed for blot development
Realistic expectation: Weeks 4-6 represent the first meaningful assessment window
