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Samples were then reduced with 10 mM dithiothreitol for 30 min at 80 C and alkylated with 20 mM iodoacetamide for 45 mins at RT and supplemented with a final concentration of 1.2% phosphoric acid and 328 L of S-Trap (Protifi) binding buffer (90% methanol, 100 mM triethylammonium bicarbonate (TEAB))
Protection by selenoprotein P in human plasma against peroxynitrite-mediated oxidation and nitration
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This was driven largely by widespread use of iron replacement as the first line therapy (71 [69%] of 103 respondents) and very low use of vitamins C or E (each first or second line at 3 [3%] units)
