For anti-C1q or anti-NeuN antibody staining, a DAKO EnVision + System (Agilent Technologies, Santa Clara, CA, USA) was used as the second antibody and the signal was detected using tyramide-enhanced fluorescein isothiocyanate (FITC), as for the TSA method
In this study, the ascorbate and glutathione content were significantly increased, which may be attributed to the increase in mRNA GR expression
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Disulfide bonds were broken using dithiothreitol, and iodoacetamide was used for reduction-alkylation
Modulation of pumpkin glutathione S-transferases by aldehydes and related compounds
Nagaya, M., Hara, H., Kamiya, T