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After three washes with PB supplemented with 0.3% Triton X-100 (PBT), the sections were incubated overnight at 4C in PBT containing 0.3% BSA (Santa Cruz), 2% normal horse serum (Vector Laboratories), and the mouse anti-NeuN and rabbit anti-TrkA or anti-NGF primary antibodies (at a dilution of 1:200 in all cases except for NeuN, which was used at a dilution of 1:600)
Biotin is a B vitamin that plays a major role in energy production by acting as a coenzyme for several carboxylase enzymes involved in the metabolism of fatty acids and amino acids
In 2011, Wang et al
The KNX Association contracted Cascoda to develop the KNX IoT open-source software stack
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