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At the end of the experiment, the cells were identified on the basis of their response to high concentrations of KCl

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S-5-HT was measured with the HPLC-ECD chromatographic method ( st and the 2 nd samples were analyzed independently, as soon as possible after each blood draw, using the same method described above

Precision was calculated as the relative standard deviation, whereas accuracy was considered as the percentage of analyte recovery using the following formula: accuracy (%) = 100% x (measured amount - endogenous content)/added amount