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PMID: 23912329

The primary inoculum was prepared by transferring 1 mL of the stock culture to 30 mL of LB medium in a 250 mL flask and culturing at 37 C for 6 h

NAD+ aids the liver in detoxification, which promotes overall health

Barretina J, Caponigro G, Stransky N, Venkatesan K, Margolin AA, Kim S, et al

Ethics statement All aspects of the animal studies and all protocols met the accepted criteria for the care and experimental use of laboratory animals, and were approved by the Animal Research Committee of the 1st Faculty of Medicine, Charles University in Prague (under registration No

Or I transfer the broth to portioned -cup and 1-cup silicone molds for freezing